Custom BioGenic Systems (CBS) Freezer
The CBS Isothermal V3000 freezer is shared by several labs in the department including the Cook, Kulhman, and Strahl labs. It is located in Room 3064 on the third floor of the Genetic Medicine Building (GMB). There is a map of the racks in this freezer on the Cook Lab whiteboard next to Team Area 3061.
The Cook lab takes primary responsibility for maintaining the supply of liquid nitrogen for the unit, but maintenance of the freezer is the responsibility of all users. If there any questions or problems, see the Cook Lab manager or another Cook Lab member.
Having the lid open uses a lot of nitrogen. So keep the lid up only a bare minimum of time: seconds, rather than minutes. Always work out exactly what you need and where it should be found before opening the unit. Note that the CryoPlus 2 is on the department’s Minus80 Monitoring temperature alarm system (see Question 10 on this website), so users can see exactly how long you have the lid raised.
Open and close the lid very slowly so that the warm air is not pulled into the freezer. This will greatly diminish the amount of fog you have to deal with inside the freezer. When closing the lid, make sure the latch is engaged. Clamp down the latch by turning the portion with the keyhole as far clockwise as it can go.
Working with liquid nitrogen can be hazardous. So do not go into a liquid nitrogen freezer unless you have been trained to do so by an experienced colleague. Please read the following document from the UNC’s Department of Environment, Health and Safety:
CyroPlus 2 Freezer
The CryoPlus 2 is a liquid nitrogen storage unit shared by many labs in the department, including the Neher and Meissner labs. It is located in Corridor 3000F on the third floor of the Genetic Medicine Building.
The Meissner Lab generously takes primary responsibility for maintaining the supply of liquid nitrogen for the unit. But maintenance of the CryoPlus 2 is the responsibility of all users.
Be especially careful to have the lid raised for the minimum amount of time necessary to retrieve or deposit samples so that minimal liquid nitrogen is lost to sublimation. Always work out exactly what you need and where it should be found before opening the unit. Note that the CryoPlus 2 is on the department’s Minus80 Monitoring temperature alarm system (see Question 10 on this website), so users can see exactly how long you have the lid raised.